A.Y. Paritova, Y.A. Balji, G.K. Murzakayeva, B.Z. Aytkozhina, A.A. Zhanabayev, G.B. Kuzembekova, K.K. Ashimova, G.A. Abulgazimova, Z.B. Kuanchaleyev, D. Askambayeva and Y. Mayer.

S. Seifullin Kazakh Agro Technical Research University, Astana, Kazakhstan

ABSTRACT 

The paper reports the results of studies on the isolation of Lactobacillus strains with probiotic properties for use as a fish feed supplement. For this purpose, a pure culture of Lactobacillus strains with probiotic activity was isolated from the intestines of fish, and molecular genetic identification of the isolated Lactobacilli was carried out by 16S RNA sequencing following the Sanger method. The amplicons of 20 bacterial samples with a fragment length of 790bp were obtained.

The results of nucleotide sequence decoding are as follows: two nucleotide sequences were not identified, and fish pathogens causing intestinal disorder belonging to three species (Kurthia gibsonii, Kurthia zopfii, and Lactococcus garvieae) were identified from eight sequences. The remaining 10 strains were identified as Lactobacillus belonging to two species: Leuconostoc mesenteroides and Lactococcus lactis. A phylogenetic tree was constructed using the nucleotide sequences. Catalase activity determination results confirm that the L. mesenteroides and L. lactis strains do not possess catalase activity. In contrast, air bubbles were formed when K. gibsonii strain was used, indicating catalase activity. These findings highlight the potential of L. mesenteroides and L. lactis as probiotic strains for developing functional fish feed supplements.

Key words: Lactobacillus Strains, Probiotic activity, Fish feed supplement, Nucleotide sequences.

Full version of the article: Isolation of Lactobacillus Strains with Probiotic Activity to be used as a Fish Feed Supplement.pdf

Suggested Citation: A.Y. Paritova, Y.A. Balji, G.K. Murzakayeva, B.Z. Aytkozhina, A.A. Zhanabayev, G.B. Kuzembekova, K.K. Ashimova, G.A. Abulgazimova, Z.B. Kuanchaleyev, D. Askambayeva and Y. Mayer. Isolation of Lactobacillus Strains with Probiotic Activity to be used as a Fish Feed Supplement. International Journal of Veterinary Science. Online First: 14-Dec-24. https://doi.org/10.47278/journal.ijvs/2024.268